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JacquelineJoey 18 Ngee Ann Poly Disclaimer In this disclaimer, I wanted to welcome you in my blog and thanks for passing by or visiting by any chance in here. This blog is purely created for our INAC project. Hoep you have a great time here! :D Tagboard Archives  Recent posts:Credits Layout: Nicole and Dirah. Resources: Soonei and Sugarpink.  |
Collection of Milk Samples & Obtaining a Representative Sample
Collection of Milk Samples & Obtaining a Representative Sample
- Recruitment of volunteer mothers into the study will be approved and certified by Institutional Research Board (IRB) Singapore. Informed consent will be sought from the volunteer mothers.
- Milk will be stored in sterile plastic containers using an electric pump from the neonatal unit. The milk obtained will be immediately frozen at -40 degree Celsius until analysis. Analysis will be done within 10 days from the collection to prevent any inaccuracy of results due to stale human milk.
- Stock solutions of δ-tocopherol standards (10mg/mL) were prepared in ethanol and kept for 1 week at a temperature of -20 degree Celsius.
-Spiking solutions of δ-tocopherol standards will be prepared on the day of analysis via dilutions from stock solutions. δ- tocopherol was used as internal standards as its chemical structure was similar to those of the other forms of tocopherol assayed (i.e. α and γ-tocopherol and tocopherol acetate).  |